Autologous 3D printed scaffolds (AutoInk)

ARI Exploratory Research
S Verrier, D Eglin, M Alini

Background

The development of 3D printing and 3-dimensional assembly of multi-cellular types technologies, parallel to the increasing need of patient-specific solutions has motivated the choice of layer-by-layer bioprinting for the fabrication of complex tissue engineered cellular scaffolds or tissues. The basic material for bio-printing usually comprises a soft biomaterial (ink) loaded with leaving cells enabling deposition in a designed shape. Optimal features for a bioink are appropriate rheological properties, printability, cytocompability for cell delivery, but also mechanical stability (maintain 3D architecture) and controlled biodegradability. Being of autologous origin, platelet rich plasma (PRP) has gained popularity in tissue repair and regeneration as potential treatment for various acute and chronic disorders in human and veterinary medicine, including tendon and craniomaxillofacial applications. PRP has proven to be an adequate extracellular matrix environment for mesenchymal stem cell proliferation and was also shown to promote vascularization when seeded with endothelial cells. Naturally rich in fibrinogen and platelets and, originally liquid PRP jellifies upon thrombin and calcium-chloride activation. The rheological properties of PRP gels are depending on its original concentration and can be modulated upon platelet activation. To date, the use of PRP in form of injectable gel or spray is widely accepted in the clinic.

A: Injectability test of PRP modified by the addition of hyaluronic acid methacrylate (HaMa), gelatin methacrylate (GelMa), or a mix of both (GelHama). B: Rheological analysis of the above mentioned PRP composites. Storage modulus (G') and loss modulus (G") as function of angular frequency shows the efficiency of cross-linking if the gels

 

Goal

To develop an autologous biological ink based on platelet rich plasma for the biofabrication of shape and biologics patient specific bone implants

  • Publication

    Herrmann M, Stanic B, Hildebrand M, Alini M, Verrier S. In Vitro Simulation of the Early Proinflammatory Phase in Fracture Healing Reveals Strong Immunomodulatory Effects of CD146-positive Mesenchymal Stromal Cells. J Tissue Eng Regen Med. 2019;13(8):1466-1481

  • Presentation

    Healing of large bone defects. S, Verrier, 4th congress Gene Therapy & Regenerative Medicine, Athens, Greece, May 2019. (invited presentation)

    Regulation of pericyte function in angiogenesis and tissue regeneration: The role for T-cadherin.

    Dasen B, Guerrero J, Scherberich A, Verrier S, Martin I, Philippova M. 2019 TERMIS EU (oral)

Completed