Tissue engineering and cell biology

Cell and tissue culture

• Cell isolation (eg, osteoblasts, chondrocytes, stem cells) from various tissues (eg, bone, cartilage, disc, veins, bone marrow, peripheral blood) and from various species (eg, human samples, bovine, sheep, rat)
• Tissue biopsies from various locations and species (eg, cartilage and intervertebral disc)
• Two-dimensional and threedimensional cell culture
• Differentiation of precursor or stem cells in specific cell types (eg, osteoblasts or chondrocytes)
• Bioreactor culture systems for intervertebral disc (organ culture) and articular cartilage (pin-on-ball)
• Gene transfer: non-viral and viral

Analysis

• Gene expression
• Isotope incorporation
• Biochemical analysis (eg, glycosaminoglycan, DNA, alkaline phosphatase)
• Cell viability
• Time lapse microscopy
• Protein analysis
• Fluorescence Activated Cell Sorting
• Biomarker screening

Polymer Processing and Characterization

• 3-D Printing, biofabrication, scaffold, film and membrane processing
• Gel permeation chromatography, viscosimetry, thermal analysis, trace analysis, in vitro degradation, contact angle, hydrolytic permeability, rheology